GC-MS analysis

Gas chromatography-mass spectrometry (GC-MS) is a popular and versatile analysis technique for identifying and quantifying concentrations of organic substances. Common applications of GC-MS include quality control in the food industry, impurity screening of environmental samples, and food contact material migration testing.

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What is GC-MS used for?

GC-MS and its high-sensitivity version, GC-MS/MS, are commonly used to screen different types of samples for volatile and semi-volatile organic contaminants, such as polycyclic aromatic hydrocarbons (PAH), phthalates, and pesticides. Applications for which Measurlabs offers GC-MS analyses include the following:

As GC-MS is an exceptionally versatile method, the above are just some examples of popular applications. Do not hesitate to contact us to discuss your analysis needs.

How does the method work?

In GC-MS, the sample is first vaporized and injected into a gas chromatography (GC) column that is coated with a stationary phase. The column is heated, and an inert gas (the mobile phase) carries the sample through it. Compounds with distinct boiling points and polarities interact with the stationary phase in different ways, which leads to their separation and elution from the column at different retention times (RT).

The separated compounds are transferred to a mass spectrometer (MS), where they are ionized and fragmented. The ions are further separated based on their mass-to-charge (m/z) ratios using a magnetic or electronic field. As a result, a mass spectrum, with peaks corresponding to different compounds, is generated. By comparing the data on retention time and (m/z) ratios to existing standards, it is possible to identify and quantify compounds present in the sample.

GC-MS diagram
A simplified diagram of a GC-MS setup.

Suitable samples for GC-MS

GC-MS is well suited for detecting various types of molecules in a wide variety of sample matrices. A requirement for GC-MS analysis is that the compounds in the sample are volatile or semi-volatile so that they can be separated from each other with a gas chromatograph and identified by a mass spectrometer. Many gaseous, liquid, and solid samples are suitable for GC-MS analysis without significant pretreatment. However, the analysis of complex samples or very small sample amounts may require more preparation.

Sample preparation

Gases and very easily volatile samples can be analyzed by collecting the substances of interest with the help of solid-phase microextraction (SPME). Then the compounds can be released into the gas chromatograph for analysis. Low-volatility compounds can be made more volatile through derivatization, where a chemical group, such as methyl or silyl, is attached to the target compound to lower its boiling point.

To examine samples that contain significant amounts of non-volatile matter, a headspace (HS) autosampler can be used. The sampler dispenses gas into the gas chromatograph from the top of the actual test sample, making it possible to analyze volatile compounds originating from non-volatile samples, such as soil or construction materials.

Yet another option is to apply the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method to complex matrices to simplify sample extraction and cleanup. The method is most commonly used to analyze pesticide residues and mycotoxins in food industry samples.

The analysis can also be started with pyrolysis, i.e. breaking the sample through exposure to heat. The resulting py-GC-MS analysis is well-suited for analyzing polymeric materials, lignin, and other complex sample types. A GC-MS setup may also be connected to a thermogravimetric analyzer to analyze the evolved gases that are formed when the sample is heated.

Need an analysis?

Measurlabs offers a comprehensive selection of purity and composition analyses using GC-MS and related methods, such as GC-ECD and GC-FID. Do not hesitate to contact us through the form below to discuss your testing needs. Our experts will get back to you within one business day.

Suitable sample matrices

  • Unknown organic materials
  • Pure chemicals and different mixtures
  • Food and beverage
  • Fuels, oils and lubricants
  • Environmental samples

Ideal uses of GC-MS

  • Analysis of unknown organic compounds and mixtures
  • Food and beverage safety testing to identify harmful compounds and determine their concentrations
  • Compositional analysis of oils, fuels, and lubricants, e.g. PAH analysis
  • Environmental tests, such as impurity screening of air, water, and soil samples

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Frequently asked questions

What is GC-MS commonly used for?

GC-MS is most commonly used for separating and identifying different compounds from a complex mixture of substances as well as determining their quantities. GC-MS can be used, for instance, to screen food products for toxins or to determine the amount of volatile organic compounds in natural gases. The method can also be used to test petroleum products in the oil refining industry to find PAHs and to perform environmental tests on water, soil, and air samples to detect POPs and other contaminants.

When the compounds contained in the sample are known and only their concentrations are to be determined, gas chromatography alone is sufficient to obtain the results. However, if the composition of the sample is unknown, mass spectrometry is needed to identify the compounds. In this case, GC-MS should be used.

What are the limitations of GC-MS analysis?

The compounds that are examined must be volatile or semi-volatile for gas chromatography to work. The sample matrix must be free of non-volatile substances, or a suitable method must be used to prevent the non-volatile matter from entering the gas chromatograph (HS-GC-MS, TD-GC-MS, SPME-GC-MS, or similar).

Liquid chromatography methods (HPLC-MS, HPLC-DAD) can be used to identify non-volatile substances that are not suited for GC-MS analysis.

What kinds of samples can be analysed using GC-MS?

GC-MS is a suitable method for analyzing gaseous, liquid, and solid samples. Gaseous and solid samples, as well as liquid samples containing non-volatile matter, require pretreatment and/or the use of a special autosampler prior to the analysis. Very complex samples containing many different substances can be analyzed with this method combination, but they must first be separated from possible non-volatiles, such as dirt.

What is Measurlabs?

Measurlabs offers a variety of laboratory analyses for product developers and quality managers. We perform some of the analyses in our own lab, but mostly we outsource them to carefully selected partner laboratories. This way we can send each sample to the lab that is best suited for the purpose, and offer high-quality analyses with more than a thousand different methods to our clients.

How does the service work?

When you contact us through our contact form or by email, one of our specialists will take ownership of your case and answer your query. You get an offer with all the necessary details about the analysis, and can send your samples to the indicated address. We will then take care of sending your samples to the correct laboratories and write a clear report on the results for you.

How do I send my samples?

Samples are usually delivered to our laboratory via courier. Contact us for further details before sending samples.