Vitamin B7 (biotin) in food and dietary supplements

Determination of vitamin B7 (biotin) in food with an HPLC-UV-VIS method, or in supplements with a UPLC-MS/MS method.

According to Regulation (EU) No 1169/2011, the biotin content of food products may be included in the nutrition declaration if it is present in significant amounts. The daily reference intake for biotin is 50 µg, and biotin content can be declared if the product contains at least:

• 7.5 µg/100 g or 100 ml in products other than beverages (15% of reference intake)

• 3.75 µg/100 ml in beverages (7.5% of reference intake)

• 7.5 µg per portion if the package contains a single portion (15% of reference intake)

Foods that contain at least the above amounts of biotin can be labeled with the nutrition claim "Source of biotin."

Additional nutrition and health claims related to vitamin B7 can be applied in accordance with Regulations (EC) No 1924/2006 and (EU) No 432/2012.