UPLC-MS analysis

Determination of vitamin B1 (thiamine) in food with an HPLC-MS/MS method, or in dietary supplements with a UPLC-MS/MS method. According to Regulation (EU) No 1169/2011, thiamine content may be included in the nutrition declaration for food products if it is present in significant amounts. Considering the daily reference intake (DRI) of 1.1 mg, thiamin can be declared in at least the following amounts: 165 µg/100 g or 100 ml in products other than beverages (15% of DRI), 82.5 µg/100 ml in beverages (7.5% of DRI), 165 µg per portion if the package contains a single portion (15% of DRI). Foods and beverages that contain at least the above amounts of thiamine are considered sources of thiamine. Such products may be labeled with additional health claims, such as: Thiamine contributes to normal energy-yielding metabolism, Thiamine contributes to normal functioning of the nervous system, Thiamine contributes to the normal function of the heart, Thiamine contributes to normal psychological function. More information on permitted nutrition and health claims can be found in Regulations (EC) No 1924/2006 and (EU) No 432/2012.

Determination of vitamin B2 (riboflavin) in food with an HPLC-MS/MS method, or in dietary supplements with a UPLC-MS/MS method. According to Regulation (EU) No 1169/2011, the daily reference intake for riboflavin is 1.4 mg. Riboflavin content may be included in the nutrition declaration if it is present in food products in at least the following amounts: 210 µg/100 g or 100 ml in products other than beverages (15% of reference intake), 105 µg/100 ml in beverages (7.5% of reference intake), 210 µg per portion if the package contains a single portion (15% of reference intake). Foods and beverages that contain at least the above amounts of riboflavin can be labeled with the nutrition claim "Source of riboflavin." Some of the health claims applicable to such products include the following: "Riboflavin contributes to normal energy-yielding metabolism", "Riboflavin contributes to the maintenance of normal skin", "Riboflavin contributes to normal functioning of the nervous system", "Riboflavin contributes to the reduction of tiredness and fatigue".

UPLC-MS/MS analysis for determining the melatonin content in dietary supplements. According to Regulation (EU) No 432/2012, melatonin supplements may be marketed with the following health claims: "Melatonin contributes to the alleviation of subjective feelings of jet lag." The claim can be used if the product contains at least 0.5 mg of melatonin per portion., "Melatonin contributes to the reduction of time taken to fall asleep." The claim can be used if the product contains at least 1 mg of melatonin per portion..

Determination of vitamin B7 (biotin) in food with an HPLC-UV-VIS method, or in supplements with a UPLC-MS/MS method. According to Regulation (EU) No 1169/2011, the biotin content of food products may be included in the nutrition declaration if it is present in significant amounts. The daily reference intake for biotin is 50 µg, and biotin content can be declared if the product contains at least: 7.5 µg/100 g or 100 ml in products other than beverages (15% of reference intake), 3.75 µg/100 ml in beverages (7.5% of reference intake), 7.5 µg per portion if the package contains a single portion (15% of reference intake). Foods that contain at least the above amounts of biotin can be labeled with the nutrition claim "Source of biotin." Additional nutrition and health claims related to vitamin B7 can be applied in accordance with Regulations (EC) No 1924/2006 and (EU) No 432/2012.

Determination of vitamin B3 as niacin, nicotinic acid, and nicotinamide in food or supplements with a high-performance liquid chromatography method. Regulation (EU) No 1169/2011 on the provision of food information to consumers states that niacin may be included in the nutrition declaration for food products if it is present in significant amounts. Considering the daily reference intake (DRI) of 16 mg, niacin content may be declared in at least the following concentrations: 2.4 mg/100 g or 100 ml in products other than beverages (15% of DRI), 1.2 mg/100 ml in beverages (7.5% of DRI), 2.4 mg per portion if the package contains a single portion (15% of DRI). The nutrition label "Source of niacin" can be applied to products that contain the vitamin in at least the above amounts. Permitted health claims applicable to products that are sources of niacin include: Niacin contributes to normal energy-yielding metabolism., Niacin contributes to normal functioning of the nervous system., Niacin contributes to normal psychological function., Niacin contributes to the reduction of tiredness and fatigue..

Determination of perfluoroalkyl compounds (PFAS) in various types of chemical samples using the LC-MS technique. We offer several analysis packages that contain selected PFAS compounds. For example, the following package can be used for most chemical samples: Abbreviation Compound CAS number PFBA perfluorobutanoic acid 375-22-4 PFPeA perfluoropentanoic acid 2706-90-3 PFHxA perfluorohexanoic acid 307-24-4 PFHpA perfluoroheptanoic acid 375-85-9 PFOA perfluorooctanoic acid 335-67-1 PFNA perfluorononanoic acid 375-95-1 PFDA perfluorodecanoic acid 335-76-2 PFUnA; PFUdA perfluoroundecanoic acid 2058-94-8 PFDoA perfluorododecanoic acid 307-55-1 PFTrDA; PFTriA perfluorotridecanoic acid 72629-94-8 PFTeA perfluorotetradecanoic acid 376-06-7 PFHxDA perfluorohexadecanoic acid 67905-19-5 PFODA perfluorooctadecanoic acid 16517-11-6 PFBS perfluorobutanesulfonic acid 375-73-5 PFPeS perfluoropentanesulfonic acid 2706-91-4 PFHxS perfluorohexanesulfonic acid 355-46-4 PFHpS perfluoroheptanesulfonic acid 375-92-8 PFOS perfluorooctanesulfonic acid 1763-23-1 PFNS Perfluorononanesulfonic acid 68259-12-1 PFDS perfluorodecanesulfonic acid 335-77-3 PFUnDS perfluoroundecanesulfonic acid 749786-16-1 PFDoS perfluorododecanesulfonic acid 79780-39-5 HFPO-DA (Gen X) 2,3,3,3-Tetrafluoro-2-(heptafluoropropoxy)propanoic acid 13252-13-6 HFPO-TA perfluoro-2,5-dimethyl-3,6-dioxanonanoic acid 13252-14-7 DONA; ADONA 4,8-dioxa-3H-perfluorononanoic acid 919005-14-4 PFMOPrA perfluoro-3-methoxypropanoic acid 377-73-1 NFDHA perfluoro-3,6-dioxaheptanoic acid 151772-58-6 PFMOBA perfluoro-4-methoxybutanoic acid 863090-89-5 PFecHS cyclohexanesulfonic acid, 1,2,2,3,3,4,5,5,6,6-decafluoro-4-(1,1,2,2,2-pentafluoroethyl)-, potassium salt (1:1) 335-24-0 3:3 FTCA 2H,2H,3H,3H-perfluorohexanoic acid 356-02-5 5:3 FTCA 2H,2H,3H,3H-perfluorooctanoic acid 914637-49-3 7:3 FTCA 2H,2H,3H,3H-perfluorodecanoic acid 812-70-4 PFEESA perfluoro(2-ethoxyethane)sulfonic acid 113507-82-7 6:2 Cl-PFESA; 9Cl-PF3ONS 9-chlorohexadecafluoro-3-oxanonane-1-sulfonic acid 756426-58-1 8:2 Cl-PFESA; 11Cl-PF3OUdS 11-chloroeicosafluoro-3-oxaundecane-1-sulfonic acid 763051-92-9 4:2 FTSA; 4:2 FTS 4:2 fluorotelomer sulfonic acid 757124-72-4 6:2 FTSA; 6:2 FTS 6:2 fluorotelomer sulfonic acid 27619-97-2 8:2 FTSA; 8:2 FTS 8:2 fluorotelomer sulfonic acid 39108-34-4 FBSA perfluorobutane sulfonamide 30334-69-1 FHxSA perfluorohexanesulfonamide 41997-13-1 FOSA perfluorooctanesulfonamide 754-91-6 MeFOSA; N-MeFOSA n-methylperfluorooctanesulfonamide 31506-32-8 EtFOSA; N-EtFOSA n-ethylperfluorooctanesulfonamide 4151-50-2 MeFOSE n-methylperfluorooctanesulfonamidoethanol 24448-09-7 EtFOSE n-ethylperfluorooctanesulfonamidoethanol 1691-99-2 NMeFOSAA; MeFOSAA n-methylperfluorooctanesulfonamidoacetic acid 2355-31-9 NEtFOSAA; EtFOSAA n-ethylperfluorooctanesulfonamidoacetic acid 2991-50-6 FOSAA perfluorooctane sulfonamidoacetic acid 2806-24-8 10:2 FTS 10:2 Fluorotelomer sulfonic acid 108026-35-3 Target compounds and reporting limits can vary depending on the sample matrix. Typical limits of reporting vary from 1 to 50 ng/L for liquid samples and 1 to 100 μg/kg for solid samples. Contact us with a description of your samples and analysis goals, so that we can confirm method suitability and prepare a quotation.

Glycoalkaloids are naturally occurring compounds formed in potatoes that pose a risk to human health when present in high concentrations. Improper storage conditions can lead to the formation of glycoalkaloids, and especially green color in potatoes can indicate elevated glycoalkaloid content. EU recommends monitoring the glycoalkaloid level of potatoes and potato products (Commission Recommendation (EU) 2022/561). According to the recommendation, the reasons behind levels above 100 mg/kg should be investigated. Several European countries also have rules on maximum glucoalkaloid content. For example, in Sweden and Finland, national legislation sets a limit of 200 mg/kg for glycoalkaloids in potatoes. With this method, α-chaconine, α-solanine, solasodine, and solanidine are quantified from potatoes and potato products.

Determination of vitamin B9 content in food or supplements. Depending on the matrix, the analysis covers the following parameters: Food: folic acid, 5-methyl-tetrahydrofolic acid, 5-formyl-tetrahydrofolic acid, and vitamin B9 as folic acid and folates. Testing is performed with an HPLC-MS method based on AOAC 2011.06., Supplements: vitamin B9 as folic acid with a UPLC-MS/MS method.. According to Regulation (EU) No 1169/2011, the daily reference intake for folic acid (folate) is 200 µg. According to the regulation, folic acid can be declared in the nutrition declaration, if it is present in at least the following amounts: 30 µg/100 g or 100 ml in products other than beverages (15% of reference value), 15 µg/100 ml in beverages (7.5% of reference value), 30 µg per portion if the package contains a single portion (15% of reference value). Foods and beverages that meet the above minimum content requirements are considered sources of folate. Such products may be labeled with additional health claims, such as: "Folate contributes to normal blood formation", "Folate contributes to maternal tissue growth during pregnancy", "Folate contributes to normal amino acid synthesis", "Folate contributes to the reduction of tiredness and fatigue". More information on permitted nutrition and health claims can be found in Regulations (EC) No 1924/2006 and (EU) No 432/2012.