Raman spectrometry

Raman spectroscopy is a non-destructive chemical analysis technique that is used for identification of chemical components in a sample. Analysis can be done to inorganic and organic samples, excluding metals or alloys. A Raman spectrometer measures changes in monochromatic light that is scattered inelastically from the sample surface. It gives complementary data to infrared spectroscopy.

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What is Raman spectrometry?

Raman spectroscopy is a non-destructive analytical technique. It is based on inelastic scattering of photons, known as Raman scattering. Raman spectrum provides information about the chemical structure, phase and polymorphy, crystallinity, and molecular interactions.

In Raman spectroscopy monochromatic light source is directed onto a sample. The photons interact with the molecules of the sample and are scattered inelastically. The scattered light is detected by an optical spectrometer. Raman spectra occur when light interacts with molecular vibrations and rotational energies in the sample. The energy of the photons can shift up or down, and these changes in wavelength are recorded from the incident light. Due to fluorescence, Raman spectroscopy is usually unsuitable for colored samples.

How does Raman spectrometry work?

Chemical bonds have their own characteristic vibration frequencies, which can be seen in the spectrum given by the method as peaks. Once the chemical bonds in the sample have been identified by the characteristic peaks, conclusions can be drawn from the sample about the chemical structure of the compound(s) it contains. The biggest weakness of Raman spectroscopy is the fluorescence present in some substances, which can obscure underlying spectral information. The fluorescence can emerge from the sample itself or from the impurities it contains.

What's the difference between Raman and FTIR?

Raman spectroscopy gives data complementary to Infrared (IR) spectroscopy. Where IR spectroscopy is sensitive to polar bonds and hetero-nuclear group vibrations, such as OH stretching in water, Raman spectroscopy on the other hand is sensitive to homo-nuclear molecular bonds, such as C - C, C = C and C ≡ C bonds. Because of this, Raman spectroscopy can be used to analyze samples in aqueous solutions, which would be hard to analyze with FTIR. Raman spectroscopy is also more sensitive to inorganic materials.

Suitable sample matrices

  • Samples can be inorganic or organic.
  • All physical states of matter: solids, powders, liquids, gels, slurries and gases.
  • Pure chemicals, mixtures and solutions
  • Proteins
  • Pigments and dyes
  • Carbides
  • Metal Oxides
  • Drug Products
  • Ceramics
  • Minerals (excluding pure metals or alloys)

Ideal uses of Raman spectroscopy

  • Raman spectroscopy is used to distinguish chemical composition, state and structure of the sample.
  • It is especially suitable for samples in aqueous solutions, non-fluorescent samples, and colourless samples.
  • Together with infrared spectroscopy, Raman spectroscopy can be used to get a fuller picture of the sample’s chemical composition.

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Frequently asked questions

What is Raman spectroscopy commonly used for?

Raman spectroscopy is used to analyse chemical compositions of organic and inorganic materials.

What are the limitations of Raman spectroscopy?

Fluorescence of impurities or of the sample itself can hide the Raman spectrum.

Some materials have a weak Raman effect, in this case, infrared spectroscopy is usually preferred.

Because the concentration of molecules is typically low in gases, analysis can be inaccurate.

Method is not suitable for metals or alloys.

What kind of samples can be analyzed with Raman spectroscopy?

Inorganic and organic samples, excluding pure metals and alloys, can be analysed with Raman spectroscopy.

All physical states of matter can be analysed. Samples can be solids, powders, liquids, gels, slurries and gases.

What is Measurlabs?

Measurlabs offers a variety of laboratory analyses for product developers and quality managers. We perform some of the analyses in our own lab, but mostly we outsource them to carefully selected partner laboratories. This way we can send each sample to the lab that is best suited for the purpose, and offer high-quality analyses with more than a thousand different methods to our clients.

How does the service work?

When you contact us through our contact form or by email, one of our specialists will take ownership of your case and answer your query. You get an offer with all the necessary details about the analysis, and can send your samples to the indicated address. We will then take care of sending your samples to the correct laboratories and write a clear report on the results for you.

How do I send my samples?

Samples are usually delivered to our laboratory via courier. Contact us for further details before sending samples.