HPLC-FLD analysis

High-performance liquid chromatography with fluorescence detection (HPLC-FLD) is a combined separation and detection technique for characterizing samples that contain compounds with fluorescent properties. The fluorescence detector is highly selective and sensitive to analytes including bisphenol A, melatonin, and thymoquinone.

Fluorescence
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Some of our HPLC-FLD services

Bisphenol A content

CEN/TS 13130-13, EN 14350-2, EN 14372, …
We offer bisphenol A (BPA) content determination for various matrices, including: Consumer goods made from plastic, elastomers, and rubber, Toys and childcare articles, Food packaging (plastic, paper, multi-materials), Food (herbs, spices, nuts, coffee, etc.). Extraction is possible with various techniques, including: Cold water extract (EN 645), hot water extract (EN 647), or solvent extract (EN 15519) from packaging containing recycled fibers for compliance according to German BfR recommendation XXXVI, Acetonitrile extract of food contact materials for compliance according to French regulation LOI n° 2012-1442, Release into aqueous simulant from child care articles for compliance according to EN 14372 and EN 14350, Release into aqueous simulant from toys for compliance according to Toy Safety Directive 2009/48/EC. For food contact materials, we also offer a low LOD method that meets the 1 μg/kg detection limit specified in Commission Regulation (EU) 2024/3190. Our experts are happy to provide a quote for BPA testing with this method.
254 €
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Vitamin content in food and dietary supplements

EN 12823-1, EN 14130, ISO 20633
We offer a range of liquid chromatography-based methods to determine the vitamin content of food and dietary supplement samples. Examples of target analytes include: Vitamin A (retinol, beta-carotene), Vitamin B1 (thiamine), Vitamin B2 (riboflavin), Vitamin B3 (niacin), Vitamin B5 (pantothenic acid), Vitamin B6 (pyridoxine), Vitamin B7 (biotin), Vitamin B9 (folic acid), Vitamin B12 (cyanocobalamin), Vitamin C (ascorbic acid), Vitamin D3 (cholecalciferol), Vitamin E (alpha-tocopherol, alpha-tocopheryl acetate), Vitamin K1 (phylloquinone), Vitamin K2 (menaquinone-4, menaquinone-7). According to EU regulations, the concentration of a vitamin can only be declared on the packaging if it is present in the food or supplement in significant amounts. For beverages, this means a minimum of 7.5% of the daily reference intake (DRI) per 100 ml of the product. For other products, the minimum concentration is 15% of DRI per 100 g. Vitamin content analysis is the recommended way to verify that the declared level is accurate, especially when a food or supplement is marketed with vitamin-related health claims. The displayed price range applies to the analysis of one vitamin in one sample. Package deals are available to determine several vitamins simultaneously and to analyze multiple samples. Please ask our experts for an offer.
100–180 €
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Prices excluding VAT.

  • Fast turnaround times
  • Personal service from method experts
  • Competitive prices
  • Result accuracy guarantee

What is HPLC-FLD used for?

HPLC-FLD is used to detect and quantify certain (usually organic) substances with fluorescent properties. Typical target analytes include vitamins and hormones, such as melatonin. They can be detected in trace quantities and quantified with accuracy and precision, which gives HPLC-FLD applications in quality assurance of pharmaceuticals, food supplements, and other products that must contain exact amounts of these substances.

Certain contaminants, such as bisphenol A (BPA), polycyclic aromatic hydrocarbons (PAHs), and aflatoxins, also have fluorescent properties and can therefore be detected with HPLC-FLD. This gives the method applications in contaminant screening of food, water, food contact materials, and other materials that must not contain such contaminants in concentrations exceeding certain limits (e.g. those defined in EU BPA regulations). Environmental samples can also be analyzed to monitor pollution levels.

How does the fluorescence detector work?

The fluorescence detector works by exposing the sample to a beam from a high-energy light source. Depending on the wavelength of light, this will cause certain compounds within the sample to ‘fluoresce’ and produce light of their own at a different wavelength. The detector monitors these emissions and collects data on the specific wavelengths and intensities, which in turn reveals the concentrations of different compounds within the sample. By combining this with the separation caused by HPLC, it is possible to accurately characterize a sample containing fluorescent chemicals.

HPLC FLD vs DAD

Diode array detection (DAD) is an alternative detection method to FLD in HPLC analysis. Unlike in FLD, DAD exposes the sample to a broad spectrum of light and measures which wavelengths are absorbed by the sample, rather than which wavelengths are produced by fluorescence. The main benefit of this is that it can be used on a far wider range of molecules, whereas FLD can only be used on those with naturally fluorescent properties. 

However, FLD offers a far more sensitive detection method, so in cases where it is applicable, it can detect lower quantities of specific compounds. Therefore, the suitable detection method will be dependent on the properties of the target analytes.

Other liquid chromatography methods may also be considered. For example, UPLC-MS/MS (ultra-performance liquid chromatography-tandem mass spectrometry) can be used for a larger array of analytes than HPLC-FLD, while also being extremely accurate and precise.

Need HPLC-FLD analyses?

Measurlabs offers laboratory testing with HPLC-FLD and other HPLC-techniques. Whether it’s just a dozen of samples or a large series, we offer competitive pricing and deliver reliable results quickly. With our experience serving 700+ satisfied customers across industries, we’ve seen it all and know how to tailor solutions to your exact needs. Reach out to us using the form below to request a quote and we will get back to you latest the next business day.

Suitable sample matrices

  • Water
  • Food and supplements
  • Pharmaceuticals
  • Soil

Ideal uses of HPLC-FLD

  • Contaminant analysis of food, water, biochemical, and environmental samples
  • BPA and PAH testing
  • Determining hormone and vitamin content in food and supplements
  • Quality control and development of pharmaceuticals

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Frequently asked questions

What is Measurlabs?

Measurlabs offers a variety of laboratory analyses for product developers and quality managers. We perform some of the analyses in our own lab, but mostly we outsource them to carefully selected partner laboratories. This way we can send each sample to the lab that is best suited for the purpose, and offer high-quality analyses with more than a thousand different methods to our clients.

How does the service work?

When you contact us through our contact form or by email, one of our specialists will take ownership of your case and answer your query. You get an offer with all the necessary details about the analysis, and can send your samples to the indicated address. We will then take care of sending your samples to the correct laboratories and write a clear report on the results for you.

How do I send my samples?

Samples are usually delivered to our laboratory via courier. Contact us for further details before sending samples.