Glycan profiling (N- and O-linked) of protein samples

Profiling of N- and O-linked glycans from protein samples by Mass Spectrometry. Analysis of N-linked glycans includes PNGase digestion of the proteins to liberate N-glycans, permethylation of N-glycans, and analysis of native and permethylated glycans by Mass Spectrometry. Analysis of O-linked glycans includes Isolation of O-glycopeptides after proteolytic digest of the protein, liberation of O-glycans by reductive β-elimination, permethylation of O-glycans, and analysis of permethylated glycans by Mass Spectrometry. The raw data for profiling is provided as an attachment to the test report.